Journal: bioRxiv

Article Title: Coupling Mechanical Regulation with Biochemical Reaction-Diffusion Circuits Yields Robust Self-Organized Pattern Formation

doi: 10.64898/2026.05.23.727407

Figure Lengend Snippet: (A) Schematics of spontaneous pattern formation by LALI (Local Activation and Long-Range Inhibition) circuit in multicellular systems. (B) Schematics of synNotch-based LALI circuit construction. synNotch ligand-receptor interactions mediate synthetic juxtacrine signaling that induces target gene expression, whereas secreted synNotch inhibitors function as diffusible inhibitory signals. Their combination enables construction of synthetic LALI circuits in mammalian cell culture. (C) Experimental workflow. 20000 cells of each Cell A and Cell B were cocultured in non-treat 96 well plates for 72 hr. Spatial patterns of induced fluorescent reporters were imaged by confocal microscope and processed to classify ON, OFF, and NO cell regions (magenta, green, and gray, respectively), to evaluate synthetic pattern generation.

Article Snippet: The basal/induced expression levels of BFP and IFP reporters were measured, and cells were single-cell sorted into 96-well plates (SH800S, SONY).

Techniques: Activation Assay, Inhibition, Targeted Gene Expression, Cell Culture, Microscopy